Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Suppression of KLF7 gene expression inhibits proliferation and induces apoptosis of hemangioma cells via NF-κB signaling pathway

Yang Wu¹, Fang Jin², He Huang¹, Shi Wang^3,4

¹Department of Dermatology; ²Department of Otolaryngology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing City, Jiangsu Province 210000; ³Department of Dermatology, Huangshi Central Hospital, Affiliated Hospital of Hubei Polytechnic University, Edong Healthcare Group, Huangshi, Hubei Province 435000; ⁴Hubei Key Laboratory of Kidney Disease Pathogenesis and Intervention, Huangshi, Hubei Province 435000, China.

For correspondence:- Shi Wang Email: shiwang2818@163.com Tel:+867143062863

Accepted: 28 June 2021 Published: 29 July 2021

Citation: Wu Y, Jin F, Huang H, Wang S. Suppression of KLF7 gene expression inhibits proliferation and induces apoptosis of hemangioma cells via NF-κB signaling pathway. Trop J Pharm Res 2021; 20(7):1351-1356 doi: 10.4314/tjpr.v20i7.5

© 2021 The authors.
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Abstract

Purpose: To assess the role of Kruppel-like factor 7 (KLF7) in hemangioma (HA) progression, KLF7 expression and regulation of downstream targets in HA tissues and hemangioma-derived endothelial cells (HemECs) have been evaluated.

Methods: Quantitative polymerase chain reaction (qPCR) and immunoblotting were performed to assess KLF7 expression in HA tissues and normal tissues. Endothelial cells were isolated from HA tissues, and Cell Counting Kit-8 assay and flow cytometry were carried out to analyze proliferation and apoptosis of the isolated HemECs. Immunoblotting was used to determine the effect of KLF7 on expression of members of nuclear factor kappa B (NF-κB) pathway in HemECs.

Results: High KLF7 expression occurred in infantile HAs during the proliferative stage. Knockdown of KLF7 expression in HemECs inhibited proliferation and induced apoptosis via suppression of NF-κB pathway (p < 0.001).

Conclusion: KLF7 is a promising therapeutic target for the treatment of HA.

Keywords: Hemangioma (HA), Kruppel-like factor 7 (KLF7), Endothelial cell, Apoptosis, NF-?B pathway